Ecotin (AA 21-162) protein (His tag)
Aperçu rapide pour Ecotin (AA 21-162) protein (His tag) (ABIN667329)
Antigène
Type de proteíne
Origine
Source
Application
Pureté
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Attributs du protein
- AA 21-162
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Purification/Conjugué
- His tag
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Séquence
- MGSSHHHHHH SSGLVPRGSH MAESVQPLEK IAPYPQAEKG MKRQVIQLTP QEDESTLKVE LLIGQTLEVD CNLHRLGGKL ENKTLEGWGY DYYVFDKVSS PVSTMMACPD GKKEKKFVTA YLGDAGMLRY NSKLPIVVYT PDNVDVKYRV WKAEEKIDNA VVR
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Attributs du produit
- Ecotin, 21-162aa, E.coli, His tag, E.coli
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 50mM NaCl, 1mM DTT, 10% glycerol
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Ecotin
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Sujet
- Ecotin (E. coli serine protease inhibitor) inhibits pancreatic serine proteases. This protein inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases. The strength of inhibition does not appear to be correlated with a particular protease specificity. Recombinant E. coli Ecotin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Poids moléculaire
- 18.3 kDa (163aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_416713
Antigène
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