MsrB (AA 1-137) protein (His tag)
Aperçu rapide pour MsrB (AA 1-137) protein (His tag) (ABIN667429)
Antigène
Type de proteíne
Origine
Source
Application
Pureté
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Attributs du protein
- AA 1-137
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Purification/Conjugué
- His tag
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Séquence
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MANKPSAEEL KKNLSEMQFY VTQNHGTEPP FTGRLLHNKR DGVYHCLICD APLFHSQTKY DSGCGWPSFY EPVSEESIRY IKDLSHGMQR IEIRCGNCDA HLGHVFPDGP QPTGERYCVN SASLRFTDGE NGEEING -
Attributs du produit
- msrB, 1-137aa, E.coli, His tag, E.coli
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Purification
- > 95% by SDS-PAGE
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 0.1M NaCl,1 mM DTT
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- MsrB
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Sujet
- MsrB, also known as, methionine sulfoxide reductase B from Escherichia coli, belongs to the msrB Met sulfoxide reductase family. E. coli msrB carries out the reduction of methionine-R-sulfoxide to methionine. This protein possess a metal binding site composed of two CXXC motifs. The bound metal (zinc or iron) may stabilize the conformation of the enzymes. Recombinant E. coli msrB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Poids moléculaire
- 17.6 kDa (157aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_416292
Antigène
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