SOD-A (AA 1-206) (Active) protein (His tag)

N° du produit ABIN667439

Cette protéine Recombinant est produite dans Escherichia coli (E. coli).

Aperçu rapide pour SOD-A (AA 1-206) (Active) protein (His tag) (ABIN667439)

Antigène: SOD-A

Type de proteíne: Recombinant

Activité biologique: Active

Origine: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Pureté: > 95 %

Détail du produit

Attributs du protein: AA 1-206

Purification/Conjugué: His tag

Séquence: MGSSHHHHHH SSGLVPRGSH MSYTLPSLPY AYDALEPHFD KQTMEIHHTK HHQTYVNNAN AALESLPEFA NLPVEELITK LDQLPADKKT VLRNNAGGHA NHSLFWKGLK KGTTLQGDLK AAIERDFGSV DNFKAEFEKA AASRFGSGWA WLVLKGDKLA VVSTANQDSP LMGEAISGAS GFPIMGLDVW EHAYYLKFQN RRPDYIKEFW NVVNWDEAAA RFAAKK

Attributs du produit: sodA, 1-206aa, E.coli, His tag, E.coli

Purification: > 95% by SDS-PAGE

Biological Activity Comment: Specific activity is > 300unit/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol, 0.1M NaCl

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: SOD-A

Autre désignation: sodA

Sujet: Superoxide dismutase, Mn, also known as sodA, is a member of the iron/manganese superoxide dismutase family. SodA destroys radicals which are normally produced within the cells and which are toxic to biological systems. It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase. Recombinant E. coli sodA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Poids moléculaire: 25.2 kDa (226aa) confirmed by MALDI-TOF

NCBI Accession: NP_418344