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GrxB (AA 1-215) protein (His tag)

Cette protéine Recombinant est produite dans Escherichia coli (E. coli).
N° du produit ABIN667442

Aperçu rapide pour GrxB (AA 1-215) protein (His tag) (ABIN667442)

Antigène

GrxB

Type de proteíne

Recombinant

Origine

E. coli

Source

  • 1
Escherichia coli (E. coli)

Application

SDS-PAGE (SDS)

Pureté

> 95 %
  • Attributs du protein

    AA 1-215

    Purification/Conjugué

    His tag

    Séquence

    MGSSHHHHHH SSGLVPRGSH MKLYIYDHCP YCLKARMIFG LKNIPVELHV LLNDDAETPT RMVGQKQVPI LQKDDSRYMP ESMDIVHYVD KLDGKPLLTG KRSPAIEEWL RKVNGYANKL LLPRFAKSAF DEFSTPAARK YFVDKKEASA GNFADLLAHS DGLIKNISDD LRALDKLIVK PNAVNGELSE DDIQLFPLLR NLTLVAGINW PSRVADYRDN MAKQTQINLL SSMAI

    Attributs du produit

    grxB, 1-215aa, E.coli, His tag, E.coli

    Purification

    > 95% by SDS-PAGE
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  • Indications d'application

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol, 50 mM NaCl

    Stock

    4 °C,-20 °C,-80 °C

    Stockage commentaire

    Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
  • Antigène

    GrxB

    Sujet

    GrxB (Glutaredoxin-2) belongs to the glutaredoxin family. Glutaredoxins are small redox enzymes of approximately one hundred amino-acid residues that use glutathione as a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. This protein involved in reducing some disulfides in a coupled system with glutathione reductase. It does not act as hydrogen donor for ribonucleotide reductase. Recombinant E. coli grxB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

    Poids moléculaire

    26.5 kDa (235aa) confirmed by MALDI-TOF

    NCBI Accession

    NP_415582
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