GrxB (AA 1-215) protein (His tag)
Aperçu rapide pour GrxB (AA 1-215) protein (His tag) (ABIN667442)
Antigène
Type de proteíne
Origine
Source
Application
Pureté
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Attributs du protein
- AA 1-215
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Purification/Conjugué
- His tag
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Séquence
- MGSSHHHHHH SSGLVPRGSH MKLYIYDHCP YCLKARMIFG LKNIPVELHV LLNDDAETPT RMVGQKQVPI LQKDDSRYMP ESMDIVHYVD KLDGKPLLTG KRSPAIEEWL RKVNGYANKL LLPRFAKSAF DEFSTPAARK YFVDKKEASA GNFADLLAHS DGLIKNISDD LRALDKLIVK PNAVNGELSE DDIQLFPLLR NLTLVAGINW PSRVADYRDN MAKQTQINLL SSMAI
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Attributs du produit
- grxB, 1-215aa, E.coli, His tag, E.coli
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Purification
- > 95% by SDS-PAGE
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol, 50 mM NaCl
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- GrxB
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Sujet
- GrxB (Glutaredoxin-2) belongs to the glutaredoxin family. Glutaredoxins are small redox enzymes of approximately one hundred amino-acid residues that use glutathione as a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. This protein involved in reducing some disulfides in a coupled system with glutathione reductase. It does not act as hydrogen donor for ribonucleotide reductase. Recombinant E. coli grxB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Poids moléculaire
- 26.5 kDa (235aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_415582
Antigène
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