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Ppa (AA 1-176) protein (His tag)

Cette protéine Recombinant est exprimée dans Escherichia coli (E. coli).
N° du produit ABIN667500

Aperçu rapide pour Ppa (AA 1-176) protein (His tag) (ABIN667500)

Antigène

Ppa

Type de proteíne

Recombinant

Origine

E. coli

Source

  • 1
Escherichia coli (E. coli)

Application

SDS-PAGE (SDS)

Pureté

> 95 %
  • Attributs du protein

    AA 1-176

    Purification/Conjugué

    His tag

    Séquence

    MSLLNVPAGK DLPEDIYVVI EIPANADPIK YEIDKESGAL FVDRFMSTAM FYPCNYGYIN HTLSLDGDPV DVLVPTPYPL QPGSVIRCRP VGVLKMTDEA GEDAKLVAVP HSKLSKEYDH IKDVNDLPEL LKAQIAHFFE HYKDLEKGKW VKVEGWENAE AAKAEIVASF ERAKNK

    Attributs du produit

    ppa, 1-176aa, E.coli, His tag, E.coli

    Purification

    > 95% by SDS-PAGE
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  • Indications d'application

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol, 50 mM NaCl

    Stock

    4 °C,-20 °C,-80 °C

    Stockage commentaire

    Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
  • Antigène

    Ppa

    Sujet

    Inorganic pyrophosphatase (ppa) belongs to the Ppase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis, as well as other biochemical transformations. Recombinant E. coli ppa protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

    Poids moléculaire

    21.9 kDa (196aa) confirmed by MALDI-TOF

    NCBI Accession

    NP_418647
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