G-CSF Protéine

N° du produit ABIN6699717

N° du produit (Fournisseur): 009-001-u93-0010

Protéine Recombinant G-CSF exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour G-CSF Protéine (ABIN6699717)

Antigène: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 95,00%

Détail du produit

N° du produit (Fournisseur): 009-001-u93-0010

Fournisseur: Rockland

Fonction: Human Granulocyte Colony Stimulating Factor Recombinant Protein

Purification: Granulocyte Colony Stimulating Factor purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of mouse NFS-60 and is typically 10-60 pg/mL.

Information d'application

Indications d'application:

Application Note: Granulocyte Colony Stimulating Factor Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Granulocyte Colony Stimulating Factor in immunological assays. Lyophilized in 20 mM acetic acid, 50 nM sodium chloride.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Concentration: 0.1 mg/mL

Buffer: Lyophilized in 20 mM acetic acid, 50 nM sodium chloride.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Autre désignation: CSF3

Sujet:

Synonyms: CSF-3, MGI-1G, GM-CSFβ, pluripoietin

Background: Granulocyte-Colony Stimulating Factor (G-CSF) is a growth factor that is considered the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, IL-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF, while in epithelial, endothelial, and fibroblastic cells, secretion of G-CSF is induced by IL-17. Human and mouse G-CSF are cross-reactive. Recombinant human G-CSF is a non-glycosylated protein, containing 175 amino acids, with a molecular weight of 18.8 kDa.

UniProt: P09919

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization