Cyclin O Protein (CCNO) (GST tag)

N° du produit ABIN6699749

N° du produit (Fournisseur): 009-001-r82s

Cette protéine Recombinant Cyclin O est exprimée dans Insect cells (Sf9).

Aperçu rapide pour Cyclin O Protein (CCNO) (GST tag) (ABIN6699749)

Antigène: Cyclin O (CCNO)

Type de proteíne: Recombinant

Origine: Humain

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Pureté: >95%

Détail du produit

Purification/Conjugué: Cette Cyclin O protéine est marqué à la GST tag.

N° du produit (Fournisseur): 009-001-r82s

Fournisseur: Rockland

Fonction: CyclinO recombinant protein-GST fusion protein

Purification: Recombinant full-length human CyclinO was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.

Information d'application

Indications d'application:

Application Note: CyclinO Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. CyclinO Protein is suitable for use in Western Blot. Expect a band approximately ~ 69 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: CyclinO Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Stock: -80 °C

Stockage commentaire: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Cyclin O (CCNO)

Autre désignation: CCNO

Sujet:

Synonyms: CCNO, CCNU, UDG2, Cyclin-O

Background: Cyclin O belongs to the highly conserved cyclin family. These family members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclin O plays an important role in the processes of transcription. Cyclin O also plays an important role in DNA repair and cell cycle progression. Cyclin O localizes exclusively in the nucleus (1). Cyclin O shows significant uracil-DNA glycosylase activity in which the activity of in vitro translated UDG2 was not as robust as that of the mitochondrial isoform of UNG (2). Cyclin O Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.

NCBI Accession: NM_021147