HSPA1AB protein (GST tag)

N° du produit ABIN6699933

N° du produit (Fournisseur): 009-001-f63s

Protéine Recombinant HSPA1AB exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour HSPA1AB protein (GST tag) (ABIN6699933)

Antigène: HSPA1AB

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Pureté: >90%

Détail du produit

Purification/Conjugué: GST tag

N° du produit (Fournisseur): 009-001-f63s

Fournisseur: Rockland

Fonction: HSP70 recombinant protein-GST fusion protein

Purification: Recombinant full-length human HSP70 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Information d'application

Indications d'application:

Application Note: HSP70 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. HSP70 Protein is suitable for use in Western Blot and ATPase Assay. Expect a band approximately ~96 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Other: ATPase Assay-User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: HSP70 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Stock: -80 °C

Stockage commentaire: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: HSPA1AB

Sujet:

Synonyms: HSPA1A, HSP72, HSPA1, HSPA1B, HSP70-1, Heat shock 70 kDa protein 1A/1B , Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP70.1/HSP70.2

Background: HSP70 is a member of the heat shock protein family and is synthesized by cells of many organisms in response to stress (1). HSP70 is found mostly but not exclusively in the nucleus of unstressed cells. For several hours after a short heat shock, however, it is strongly concentrated in nucleoli (2). Nucleoli are transiently damaged by such a heat shock: their morphology changes and assembly and export of ribosomes is blocked for several hours. HSP70 helps to stabilize the morphological changes of the nucleoli. HSP70 Protein is ideal for investigators involved in Signaling Proteins, Cell Stress & Chaperone Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cellular Stress, and Inflammation research.

NCBI Accession: NM_005345