IFNG1-2 Protéine

N° du produit ABIN6699981

N° du produit (Fournisseur): 012-001-v20-0020

Protéine Recombinant IFNG1-2 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IFNG1-2 Protéine (ABIN6699981)

Antigène: IFNG1-2 (Interferon, gamma 1-2 (IFNG1-2))

Type de proteíne: Recombinant

Origine: Rat

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 95,00%

Détail du produit

N° du produit (Fournisseur): 012-001-v20-0020

Fournisseur: Rockland

Fonction: Rat Interferon gamma Recombinant Protein

Purification: Interferon gamma purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined in a viral challenge assay using EMC virus on L929 and is typically less than 0.1 ng/mL.

Information d'application

Indications d'application:

Application Note: Interferon-gamma Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interferon-gamma in immunological assays. Lyophilized in 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Concentration: 0.1 mg/mL

Buffer: Lyophilized in 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: IFNG1-2 (Interferon, gamma 1-2 (IFNG1-2))

Autre désignation: INF gamma

Sujet:

Synonyms: Type II interferon, T cell interferon, Immune Interferon, MAF

Background: Interferon gamma, IFNγ, is a major immune-modulating molecule produced mainly by T cells and natural killer cells activated by antigens, mitogens or alloantigens. Most immune cells express IFNγ receptors and respond to IFNγ-induced signaling by up-regulating MHC class I expression. Recombinant rat IFNγ is a non-glycosylated protein, containing 135 amino acids, with a molecular weight of 15.6 kDa.

UniProt: P01581