MyI9 protein (GST tag)

N° du produit ABIN6700272

N° du produit (Fournisseur): 010-001-s38

Protéine Recombinant MyI9 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour MyI9 protein (GST tag) (ABIN6700272)

Antigène: MyI9

Type de proteíne: Recombinant

Origine: Souris

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Pureté: >95%

Détail du produit

Purification/Conjugué: GST tag

N° du produit (Fournisseur): 010-001-s38

Fournisseur: Rockland

Fonction: LC20 recombinant protein-GST fusion protein

Purification: Recombinant full-length mouse LC20 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.

Information d'application

Indications d'application:

Application Note: LC20 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. LC20 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~45 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Other: Kinase Assay-User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: LC20 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Stock: -80 °C

Stockage commentaire: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: MyI9

Sujet:

Synonyms: MYL9, MGC3505, MLC2, MRLC1, MYRL2, Myosin regulatory light polypeptide 9, Myosin regulatory light chain 2, smooth muscle isoform, Myosin regulatory light chain 9

Background: LC20 is the myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads (1). LC20 protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for LC20 and the deduced 172-amino acid protein is highly conserved, with only 3 differences between the human and chicken proteins. Light chain phosphorylation causes the folded monomeric form of myosin to extend and assemble into filaments. This observation established the involvement of the LC20 in conformational transitions of smooth muscle myosin (2). LC20 Protein is ideal for investigators involved in Signaling Reagents, Protein Substrates, and Cardiovascular Disease research.