NAMPT Protéine
Aperçu rapide pour NAMPT Protéine (ABIN6700313)
Antigène
Voir toutes NAMPT ProtéinesType de proteíne
Origine
Source
Application
Pureté
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N° du produit (Fournisseur)
- 009-001-w40-0005
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Fournisseur
- Rockland
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Fonction
- Human Visfatin Recombinant Protein
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Purification
- Visfatin purity was determined to be greater than 90% as determined by reducing and non-reducing SDS-pAGE.
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niveau d'endotoxine
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by its ability to induce IL-6, IL-1 beta and TNF alpha production from human PBMCs at 100 ng/mL.
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Indications d'application
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Application Note: Visfatin Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Visfatin in immunological assays.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution_Buffer: 0.02M HCl
Reconstitution_Volume: 5 μL (5-50 μL)
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Buffer
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
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Agent conservateur
- Without preservative
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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Date de péremption
- 6 months
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- NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))
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Autre désignation
- NAMPT
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Sujet
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Synonyms: Pre-B-cell colony-enhancing factor 1, PBEF
Background: Visfatin is an adipokine produced by visceral adipose tissue. Other adipokines include leptin, adiponectin, resistin, chemerin, omentin and vaspin. Visfatin has been shown to act as a pro-inflammatory factor for PBMCs and may mimic the effects of insulin on adipocytes, monocytes and hepatocytes. Recombinant human Visfatin is a non-glycosylated protein, containing 466 amino acids, with a molecular weight of 52.6 kDa.
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UniProt
- P43490
Antigène
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