PDGF Protéine

N° du produit ABIN6700393

N° du produit (Fournisseur): 009-f01-w10-0100

Cette protéine Recombinant est produite dans Escherichia coli (E. coli).

Aperçu rapide pour PDGF Protéine (ABIN6700393)

Antigène: PDGF

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 95,00%

Classe de qualité: Animal-Free

Détail du produit

N° du produit (Fournisseur): 009-f01-w10-0100

Fournisseur: Rockland

Fonction: Human Platelet Derived Growth Factor-AB Recombinant Protein (Animal Free)

Purification: Platelet-Derived Growth Factor is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-PAGE.

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of mouse 3T3 indicator cells and is typically less than 1 ng/mL.

Information d'application

Indications d'application:

Application Note: Platelet Derived Growth Factor-AB Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Platelet Derived Growth Factor-AB in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: PDGF

Autre désignation: PDGFA/PDGFB

Sujet:

Synonyms: PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, proto-oncogene c-Sis

Background: Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF. Recombinant human PDGF-AB is a non-glycosylated, disulfide-linked heterodimer, containing one 14.3 kDa alpha-chain and one 12.1 kDa beta-chain, with total molecular weight of 26.4 kDa.

UniProt: P04085