SIRT2 Protein (GST tag)

N° du produit ABIN6700529

N° du produit (Fournisseur): 009-001-t32s

Cette protéine Recombinant SIRT2 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour SIRT2 Protein (GST tag) (ABIN6700529)

Antigène: SIRT2 (Sirtuin 2 (SIRT2))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Pureté: >85%

Détail du produit

Purification/Conjugué: Cette SIRT2 protéine est marqué à la GST tag.

N° du produit (Fournisseur): 009-001-t32s

Fournisseur: Rockland

Fonction: SIRT2 recombinant protein-GST fusion protein

Purification: Full-length recombinant human SIRT2 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.

Information d'application

Indications d'application:

Application Note: SIRT2 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. SIRT2 Protein is suitable for use in Western Blot. Expect a band approximately ~64 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: SIRT2 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Stock: -80 °C

Stockage commentaire: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: SIRT2 (Sirtuin 2 (SIRT2))

Autre désignation: SIRT2

Sujet:

Synonyms: Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIRT2, SIR2L, SIR2L2, Sirtuin 2

Background: SIRT2 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT2 being a member of class I. Inhibition of SIRT2 can lead to neuroprotection in cellular and invertebrate models of Huntington's disease (1). Huntington's disease is characterized by increased sterols synthesis in neuronal cells and this process is reversed by SIRT2 inhibition. SIRT2 can deacetylate lys40 of alpha-tubulin both in vitro and in vivo (2). Knockdown of SIRT2 via small interfering RNA results in tubulin hyperacetylation. SIRT2 Protein is ideal for investigators involved in Signaling Proteins, Deacetylase/Demethylase Proteins, Cancer, Cell Cycle, and Inflammation research.

NCBI Accession: NM_030593