SIRT4 Protein (GST tag)

N° du produit ABIN6700533

N° du produit (Fournisseur): 009-001-t34s

Protéine Recombinant SIRT4 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour SIRT4 Protein (GST tag) (ABIN6700533)

Antigène: SIRT4 (Sirtuin 4 (SIRT4))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Pureté: >70%

Détail du produit

Purification/Conjugué: Cette SIRT4 protéine est marqué à la GST tag.

N° du produit (Fournisseur): 009-001-t34s

Fournisseur: Rockland

Fonction: SIRT4 recombinant protein-GST fusion protein

Purification: Full-length recombinant human SIRT4 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.

Information d'application

Indications d'application:

Application Note: SIRT4 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. SIRT4 Protein is suitable for use in Western Blot. Expect a band approximately ~62 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: SIRT4 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Stock: -80 °C

Stockage commentaire: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: SIRT4 (Sirtuin 4 (SIRT4))

Autre désignation: SIRT4

Sujet:

Synonyms: IR2L4, MGC57437, MGC130046, MGC130047, sirtuin 4, NAD-dependent protein deacetylase sirtuin-4, NAD-dependent ADP-ribosyltransferase sirtuin-4, Regulatory protein SIR2 homolog 4, SIR2-like protein 4, SIRT4, SIR2L4

Background: SIRT4 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT4 being a member of class IV. SIRT4 lacks deacetylase activity but has ADP-ribosyltransferase activity (1). Immunoprecipitation analysis shows that SIRT4 interacts with the mitochondrial enzyme glutamate dehydrogenase (GDH), and functional analysis show that SIRT4 ADP-ribosylates and inhibits GDH (2). Downregulation of SIRT4 by RNA interference activates GDH, thereby upregulating insulin secretion in response to glucose and amino acids. SIRT4 Protein is ideal for investigators involved in Signaling Proteins, Deacetylase/Demethylase Proteins, Cancer, Cell Cycle, and Inflammation research.

NCBI Accession: NM_012240

Pathways: Negative Regulation of Hormone Secretion