Vimentin Protein (VIM) (His tag)

N° du produit ABIN6700879

N° du produit (Fournisseur): 009-001-e54s

Cette protéine Recombinant Vimentin est produite dans Escherichia coli (E. coli).

Aperçu rapide pour Vimentin Protein (VIM) (His tag) (ABIN6700879)

Antigène: Vimentin (VIM)

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Pureté: >95%

Détail du produit

Purification/Conjugué: Cette Vimentin protéine est marqué à la His tag.

N° du produit (Fournisseur): 009-001-e54s

Fournisseur: Rockland

Fonction: Vimentin recombinant protein-HIS Epitope

Purification: Recombinant full-length human Vimentin was expressed by E. coli cells using an N-terminal his epitope. The purity was determined to be >95% by densitometry.

Information d'application

Indications d'application:

Application Note: Vimentin Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol. Vimentin Protein is suitable for use in Western Blot. Expect a band approximately ~60 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: Vimentin Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.

Stock: -80 °C

Stockage commentaire: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Vimentin (VIM)

Autre désignation: VIM

Sujet:

Synonyms: VIM, FLJ36605, Vimentin

Background: Vimentin is a member of intermediate filament family of proteins and is an important structural feature of eukaryotic cells (1). Along with microtubules and actin microfilaments, Vimentin make up the cytoskeleton component of cells. Studies have shown that Vimentin is attached to the nucleus, endoplasmic reticulum and mitochondria, either laterally or terminally. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol (2). Thus, Vimentin plays a key role in maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Vimentin Protein is ideal for investigators involved in Signaling Proteins, Microtubule/Actin Associated Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Inflammation, Invasion/Metastasis, and Neurobiology research.

NCBI Accession: NM_003380

Pathways: Caspase Cascade in Apoptosis