EBI3 Protéine

N° du produit ABIN6700896

N° du produit (Fournisseur): 009-001-b66

Protéine Recombinant EBI3 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour EBI3 Protéine (ABIN6700896)

Antigène: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 90,00%

Détail du produit

N° du produit (Fournisseur): 009-001-b66

Fournisseur: Rockland

Fonction: EBI-3 Human Recombinant Protein

Purification: Recombinant protein corresponds to amino acids 21 to 229 of mature human EBI3. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.

niveau d'endotoxine: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: Assay data for Human recombinant EBI-3 is based upon qualitative binding to anti-EBI-3 antibody.

Information d'application

Indications d'application:

Application Note: EBI-3 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-EBI-3 in immunological assays. The product is lyophilized from a sterile filtered aqueous solution of 0.1 % TFA and 0.5 % mannitol.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: 0.5 % Mannitol

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Autre désignation: IL-27B

Sujet:

Synonyms: Epstein-Barr virus induced 3 protein, Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B cytokine, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI-3, EBI3, IL-35, IL35

Background: Epstein-Barr Virus Induced Gene-3 (EBI-3), is a secreted glycoprotein belonging to the hematopoietin receptor family and related to the p40 subunit of IL-12. It was identified by its induced expression in B-lymphocytes in response to Epstein-Barr virus infection. EBI-3 forms heterodimers with p28 to form IL-27 and with p35 to form IL-35. Both IL-27 and IL-35 have anti-inflammatory and regulatory activity. Recombinant Human EBI is a non-glycosylated polypeptide chain consisting of 209 amino acids with a molecular weight of 23.3 kDa.

UniProt: Q14213