SARS-CoV-2 Spike S1 Protein (RBD, V367F) (His tag)

N° du produit ABIN6952653

Cette protéine Recombinant SARS-CoV-2 Spike S1 est exprimée dans HEK-293 Cells et a été mentionnée dans 1 publication.

Aperçu rapide pour SARS-CoV-2 Spike S1 Protein (RBD, V367F) (His tag) (ABIN6952653)

Antigène: SARS-CoV-2 Spike S1

Type de proteíne: Recombinant

Origine: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Application: SDS-PAGE (SDS), ELISA

Pureté: >95 % as determined by SDS-PAGE.

Détail du produit

Attributs du protein: RBD, V367F

Purification/Conjugué: Cette SARS-CoV-2 Spike S1 protéine est marqué à la His tag.

Fonction: SARS-CoV-2 (COVID-19) S protein RBD (V367F), His Tag

Séquence: AA 319-541

Attributs du produit: SARS-CoV-2 S protein RBD (V367F), His Tag (SPD-S52H4) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541 (Accession # QHD43416.1 (V367F). Predicted N-terminus: Arg 319
This protein carries a polyhistidine tag at the C-terminus.
The V367F mutation in the RBD of Spike protein shows a higher binding affinity to human ACE2.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.

Référence

Guo, Huang, Zhang, Yao, Zhou, Shen, Shen, Li, Li, Zhang, Chen, Chen, Wu, Fu, Zeng, Feng, Pi, Wang, Zhou, Lu, Li, Fang, Lu, Hu, Wang, Zhang, Gao, Adrian, Wang, Yu, Peng, Gabibov, Min, Wang, Huang et al.: "A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes. ..." dans: Nature communications, Vol. 12, Issue 1, pp. 2623, (2021) (PubMed).

Détail du antigène

Antigène: SARS-CoV-2 Spike S1

Classe de substances: Viral Protein

Sujet: It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Poids moléculaire: 27.0 kDa

ID gène: 43740568

UniProt: P0DTC2