PD-L1 Protein (His-Avi Tag,Biotin)

N° du produit ABIN7199143

Cette protéine Recombinant PD-L1 est exprimée dans HEK-293 Cells.

Aperçu rapide pour PD-L1 Protein (His-Avi Tag,Biotin) (ABIN7199143)

Antigène: PD-L1 (CD274 (PD-L1))

Type de proteíne: Recombinant

Origine: Cynomolgus, Macaque rhésus

Source: HEK-293 Cells

Pureté: >90 % as determined by SDS-PAGE.

Classe de qualité: MALS verified

Détail du produit

Purification/Conjugué: Cette PD-L1 protéine est marqué à la His-Avi Tag,Biotin.

Fonction: Biotinylated Cynomolgus / Rhesus macaque PD-L1 / B7-H1 Protein, His,Avitag™ (MALS verified)

Séquence: Phe 19 - Arg 238

Attributs du produit: Biotinylated Cynomolgus / Rhesus macaque PD-L1, His,Avitag (PDL-C82E8) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # G7PSE7-1).

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Indications d'application: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 28.8 kDa. The protein migrates as 32-40 kDa under reducing (R) condition due to glycosylation.

Commentaires:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Stock: -20 °C

Stockage commentaire: -20°C

Détail du antigène

Antigène: PD-L1 (CD274 (PD-L1))

Autre désignation: PD-L1 / B7-H1

Sujet: Synonyms: PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1,
Description: Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.

Poids moléculaire: 28.8 kDa

Pathways: Cancer Immune Checkpoints