CD99 Protein (CD99) (His-Avi Tag,Biotin)

N° du produit ABIN7199186

Cette protéine Recombinant CD99 est exprimée dans HEK-293 Cells.

Aperçu rapide pour CD99 Protein (CD99) (His-Avi Tag,Biotin) (ABIN7199186)

Antigène: CD99

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Pureté: >90 % as determined by SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette CD99 protéine est marqué à la His-Avi Tag,Biotin.

Fonction: Biotinylated Human CD99 / MIC2 Protein, His,Avitag™

Séquence: Asp 23 - Asp 122

Attributs du produit: Biotinylated Human CD99, His,Avitag (CD9-H82E7) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 122 (Accession # P14209-1).

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Indications d'application: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 13.7 kDa. The protein migrates as 22 kDa and 27 kDa under reducing (R) condition due to glycosylation.

Commentaires:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Stock: -20 °C

Stockage commentaire: -20°C

Détail du antigène

Antigène: CD99

Autre désignation: CD99 / MIC2

Classe de substances: Viral Protein

Sujet: Synonyms: CD99,HBA71,MIC2,MIC2X,MIC2Y,MSK5X,12E7,
Description: CD99 antigen is also known as MIC2, single-chain type-1 glycoprotein, HBA71, MIC2X, MIC2Y, MSK5X, 12E7, which belongs to the CD99 family. CD99 / MIC2 is expressed on all leukocytes but highest on thymocytes. Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 / MIC2 plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. CD99 acts at the same site as, but independently of PECAM1. There is also experimental evidence that it binds to cyclophilin A.

Poids moléculaire: 13.7 kDa