SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (B.1.617.2 - delta plus, Trimer) (His tag)

N° du produit ABIN7121329

Protéine Recombinant SARS-CoV-2 Spike exprimée dans HEK-293 Cells.

Aperçu rapide pour SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (B.1.617.2 - delta plus, Trimer) (His tag) (ABIN7121329)

Antigène: SARS-CoV-2 Spike (SARS-CoV-2 S)

Type de proteíne: Recombinant

Origine: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Delta plus

Source: HEK-293 Cells

Pureté: >95 % as determined by SDS-PAGE.

Classe de qualité: MALS verified

Détail du produit

Attributs du protein: B.1.617.2 - delta plus, Trimer

Purification/Conjugué: Cette SARS-CoV-2 Spike protéine est marqué à la His tag.

Fonction: SARS-CoV-2 Spike Trimer (T19R, V70F, FR157-158Del, A222V, W258L, K417N, L452R, T478K, D614G, P681R, D950N), His Tag (MALS verified)

Séquence: Val 16 - Pro 1213

Specificité: SARS-CoV-2 Spike Trimer (T19R, V70F, FR157-158Del, A222V, W258L, K417N, L452R, T478K, D614G, P681R, D950N)

Attributs du produit: SARS-CoV-2 Spike Trimer, His Tag (SPN-C52Ht) is the ectodomain of SARS-CoV-2 spike protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). Mutations T19R, V70F, FR157-158Del, A222V, W258L, K417N, L452R, T478K, D614G, P681R, D950N were identified in the SARS-CoV-2 Delta sub-lineages (Pango lineage: AY.1/AY.2/AY.3, also known as "Delta Plus"). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Indications d'application: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 137.8 kDa. The protein migrates as kDa under reducing (R) condition due to glycosylation.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS

Stock: -20 °C

Stockage commentaire: -20°C

Détail du antigène

Antigène: SARS-CoV-2 Spike (SARS-CoV-2 S)

Autre désignation: SARS-CoV-2 Spike

Classe de substances: Viral Protein

Sujet: Synonyms: Spike,S protein,Spike glycoprotein,S glycoprotein,
Description: It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Poids moléculaire: 137.8 kDa