LRRC32&TGF-beta protein (His tag)

N° du produit ABIN7125972

Cette protéine Recombinant LRRC32&TGF-beta est produite dans HEK-293 Cells.

Aperçu rapide pour LRRC32&TGF-beta protein (His tag) (ABIN7125972)

Antigène: LRRC32&TGF-beta

Type de proteíne: Recombinant

Origine: Cynomolgus

Source: HEK-293 Cells

Pureté: >90 % as determined by SDS-PAGE.

Détail du produit

Purification/Conjugué: His tag

Fonction: Cynomolgus LRRC32&TGF-beta 1 Heterotrimer Protein, His Tag&Tag Free

Séquence: Ala 18 -Asn 627 (LRRC32) & Leu 30 - Ser 390 (TGF-beta 1)

Attributs du produit: Cynomolgus LRRC32&TGF-beta 1 Heterotrimer Protein, His Tag&Tag Free is expressed from human 293 cells (HEK293). It contains AA Ala 18 -Asn 627 (LRRC32) & Leu 30 - Ser 390 (TGF-beta 1) (Accession # A0A2K5X2X9-1 (LRRC32) & A0A2K5TJB2-1 (TGF-beta 1).

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Indications d'application: Cynomolgus LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free (the molar ratio of LRRC32 & 1 equals 1:2) is produced by co-expression of LRRC32 and 1, which has a calculated MW of 68.1 kDa (LRRC32), 28.5 (LAP) and 12.8 kDa (mature 1) respectively. LRRC32 is fused with a polyhistidine tag at the C-terminus and 1 contains no tag. The reducing (R) Heterotrimer protein migrates as 70 kDa (LRRC32), 37-43 kDa (LAP) and 13 kDa (mature 1) due to glycosylation respectively.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Stock: -20 °C

Stockage commentaire: -20°C

Détail du antigène

Antigène: LRRC32&TGF-beta

Sujet: Synonyms: LRRC32 & TGF-beta 1,LRRC32&TGFB1,
GARP (LRRC32) is a transmembrane protein that binds latent-TGF-β1 and tethers it on the Treg surface. and has been proved to promote the activation and secretion of transforming growth factor β (TGF-β). The expression of GARP is highly on the surface activated Tregs and increases the suppressive function of Tregs. Additionally, GARP can bind to latent transforming growth factor β (TGF-β), thus promoting secretion and activation of TGF-β. TGF-β plays a critical rule for homeostasis and function of Tregs. Notably, it has been also observed that fibroblasts and endothelial cell lines that express GARP/latent TGF-β1 complexes do not activate TGF-β1. However, it cannot be excluded that specific stimuli are required to trigger TGF-β1 activation from complexes on the surface of these cell types.

Poids moléculaire: 68.1 kDa (LRRC32) & 41.2 kDa (TGF-beta 1)