AACS Protein (His tag)

N° du produit ABIN7317651

Cette protéine Recombinant AACS est exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour AACS Protein (His tag) (ABIN7317651)

Antigène: AACS (Acetoacetyl-CoA Synthetase (AACS))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Pureté: > 96 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette AACS protéine est marqué à la His tag.

Fonction: Recombinant Human AACS/Acetoacetyl-CoA Synthetase Protein (His Tag)

Séquence: Met 1-Phe 672

Attributs du produit: A DNA sequence encoding the full length of human AACS isoform a (NP_076417.2) (Met 1-Phe 672) was expressed, with a polyhistidine tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: AACS (Acetoacetyl-CoA Synthetase (AACS))

Autre désignation: AACS/Acetoacetyl-CoA Synthetase

Sujet: ACSF1,SUR-5,Acetoacetyl-CoA Synthetase (AACS) is a novel cytosolic ketone body (acetoacetate)-specific ligase. The AACS in adipose tissue plays an important role in utilizing ketone body for the fatty acid-synthesis during adipose tissue development. It had been improved that Acetoacetyl-CoA Synthetase is an essential enzyme for the synthesis of fatty acid and cholesterol from ketone bodies, was found to be highly expressed in mouse adipose tissue, and GC box and C/EBPs motif were crucial for AACS promoter activity in 3T3-L1 adipocytes. Moreover, AACS promoter activity was controlled mainly by C/EBPalpha during adipogenesis. AACS gene expression is particularly abundant in white adipose tissue, as it is induced during adipocyte differentiation. The human AACS promoter is a PPARgamma target gene and that this nuclear receptor is recruited to the AACS promoter by direct interaction with Sp1 (stimulating protein-1). The Acetoacetyl-CoA Synthetase has important roles in the regulation of ketone body utilization in rat liver and that these hypocholesterolemic agents have the ability to remedy the impaired utilization of ketone bodies under the diabetic condition.

Poids moléculaire:

Calculated MW: 77.0 kDa

Observed MW: 60 kDa

ID gène: 65985

NCBI Accession: NP_076417

UniProt: Q86V21

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis