BTD Protein (His tag)
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- Antigène Voir toutes BTD Protéines
- BTD (Biotinidase (BTD))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette BTD protéine est marqué à la His tag.
- Fonction
- Recombinant Human Biotinidase/BTD Protein (His Tag)(Active)
- Séquence
- Met 1-Asp543
- Attributs du produit
- A DNA sequence encoding the human BTD (P43251) (Met1-Asp543) with a C-terminal polyhistidine tag was expressed.
- Pureté
- > 95 % as determined by reducing SDS-PAGE.
- niveau d'endotoxine
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to hydrolyze biocytin to lysine and biotin. The specific activity is >500pmol/min/μg.
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Biotinidase (BTD) (AA 1-543) protein (GST tag)
BTD Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AA, AP
Biotinidase (BTD) (AA 42-543) protein (His tag)Crystallography grade BTD Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Biotinidase (BTD) (AA 22-520) protein (His tag)Crystallography grade BTD Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Biotinidase (BTD) protein (Myc-DYKDDDDK Tag)BTD Origine: Humain Hôte: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Biotinidase (BTD) (AA 44-545) protein (His tag)BTD Origine: Humain Hôte: Baculovirus infected Insect Cells Recombinant > 85 % by SDS - PAGE SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Antigène
- BTD (Biotinidase (BTD))
- Autre désignation
- Biotinidase/BTD (BTD Produits)
- Synonymes
- BTD Protein, CG3599 Protein, CT12113 Protein, Dmel\\CG3599 Protein, zgc:153333 Protein, Biotinase Protein, Biotinidase Protein, biotinidase Protein, Btnd Protein, btd Protein, BTD Protein, Btd Protein
- Sujet
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Background: Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing of fats, carbohydrates, and proteins. Biotin is attached to these carboxylase enzymes through an amino acid (the building material of proteins) called lysine, forming a complex calledbiocytin.
Synonym: BTD
- Poids moléculaire
- 58.2 kDa
- UniProt
- P43251
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