BTD Protein (His tag)

N° du produit ABIN7194393

Cette protéine Recombinant BTD est produite dans HEK-293 Cells.

Aperçu rapide pour BTD Protein (His tag) (ABIN7194393)

Antigène: BTD (Biotinidase (BTD))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette BTD protéine est marqué à la His tag.

Fonction: Recombinant Human Biotinidase/BTD Protein (His Tag)

Séquence: Met 1-Asp543

Attributs du produit: A DNA sequence encoding the human BTD (P43251) (Met1-Asp543) with a C-terminal polyhistidine tag was expressed.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to hydrolyze biocytin to lysine and biotin. The specific activity is > 500pmol/min/μg.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: BTD (Biotinidase (BTD))

Autre désignation: Biotinidase/BTD

Sujet: BTD,Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing offats,carbohydrates, andproteins. Biotin is attached to these carboxylase enzymes through anamino acid(the building material of proteins) calledlysine, forming a complex calledbiocytin.

Poids moléculaire:

Calculated MW: 58.2 kDa

Observed MW: 66-76 kDa

ID gène: 686

UniProt: P43251