Butyrylcholinesterase Protein (BCHE) (His tag)

N° du produit ABIN7194449

Protéine Recombinant Butyrylcholinesterase exprimée dans HEK-293 Cells.

Aperçu rapide pour Butyrylcholinesterase Protein (BCHE) (His tag) (ABIN7194449)

Antigène: Butyrylcholinesterase (BCHE)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 97 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette Butyrylcholinesterase protéine est marqué à la His tag.

Fonction: Recombinant Human Butyrylcholinesterase/BCHE Protein (His Tag)(Active)

Séquence: Met 1-Leu 602

Attributs du produit: A DNA sequence encoding the human BCHE (NP_000046.1) (Met 1-Leu 602) was expressed, fused with a polyhistidine tag at the C-terminus.

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave Butyrylthiocholine. The specific activity is >50,000 pmoles/min/μg.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: Butyrylcholinesterase (BCHE)

Autre désignation: Butyrylcholinesterase/BCHE

Sujet:

Background: Butyrylcholinesterase (BCHE), also known as cholinesterase or BuChE, is an enzyme defined as "pseudo" or "non-neuronal" cholinesterase. Butyrylcholinesterase (BCHE) is widely distributed in the nervous system as well as blood plasma. It is constitutively similar to the neuronal acetylcholinesterase, and is a non-specific cholinesterase which hydrolyses many different choline esters. Butyrylcholinesterase (BCHE) is a glycoprotein of 4 identical subunits, that were arranged as a dimer of dimers with each dimer composed of two identical subunits joined by interchain disulfide bonds. Butyrylcholinesterase (BCHE) behaves principally similar to the true enzyme and thus can play a similar role in nerve conduction, although it participates probably only in relatively slow conductive processes and could be involved in other nervous system functions and in neurodegenerative diseases. It can hydrolyze toxic esters such as cocaine or scavenge organophosphorus pesticides and nerve agents. Purified human serum cholinesterase combines in its active surface an anionic and an esteratic site, similar to true cholinesterase. It has been demonstrated that butyrylcholinesterase (BCHE) may have a greater role in cholinergic transmission than previously surmised, making BChE inhibition an important therapeutic goal in Alzheimer's disease.

Synonym: Cholinesterase; Acylcholine Acylhydrolase; Butyrylcholine Esterase; Choline Esterase II; Pseudocholinesterase; BCHE; CHE1

Poids moléculaire: 66.5 kDa

NCBI Accession: NP_000046

Pathways: Peptide Hormone Metabolism