CAMK1G Protein (His-GST)

N° du produit ABIN7317051

Protéine Recombinant CAMK1G exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour CAMK1G Protein (His-GST) (ABIN7317051)

Antigène: CAMK1G (Calcium/calmodulin-Dependent Protein Kinase IG (CAMK1G))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Pureté: > 85 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette CAMK1G protéine est marqué à la His-GST.

Fonction: Recombinant Human CAMK1G/CaMKI gamma Protein (His & GST Tag)

Séquence: Met 1-Met 476

Attributs du produit: A DNA sequence encoding the human CAMK1G isoform 1 (Q96NX5-1) (Met 1-Met 476) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as sterile solution of 50 mM Tris, 100 mM NaCl, pH 8.0, 20 % glycerol, 0.3 mM DTT

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: CAMK1G (Calcium/calmodulin-Dependent Protein Kinase IG (CAMK1G))

Autre désignation: CAMK1G/CaMKI gamma

Sujet: CLICK3;CLICKIII;RP1-272L16.2;VWS1;dJ272L16.1,Calmodulin-Dependent Protein Kinase (CaM Kinase) is a kind of protein phosphorylate multiple downstream targets. Concentration of cytosolic calcium functions as a second messenger that mediates a wide range of cellular responses. Calcium binds to calcium binding proteins (calmodulin/CaM) and stimulates the activity of a variety of enzymes, including CaM kinases referred to as CaM-kinases (CaMKs), such as CaMKI, CaMKII, CaMKIV and CaMKK. Calmodulin-dependent protein kinase CL3/CaMKIγ is a memberane-anchored CaMK belonging to the CaM kinase family. Its C-terminal region is uniquely modified by two sequential lipidification steps: prenylation followed by a kinase-activity-regulated palmitoylation. These modifications are essential for CaMKIγ membrane anchoring and targeting into detergent-resistant lipid microdomains in the dendrites. It has been found that CaMKIγ critically contributed to BDNF-stimulated dendritic growth. Raft insertion of CaMKIγ specifically promoted dendritogenesis of cortical neurons by acting upstream of RacGEF STEF and Rac, both present in lipid rafts. Thus, CaMKIγ may represent a key element in the Ca2+-dependent and lipid-raft-delineated switch that turns on extrinsic activity-regulated dendrite formation in developing cortical neurons.

Poids moléculaire:

Calculated MW: 81.0 kDa

Observed MW: 75 kDa

ID gène: 57172

UniProt: Q96NX5