CMBL Protein (His tag)

N° du produit ABIN7317566

Détail du produit

Antigène: CMBL (Carboxymethylenebutenolidase Homolog (CMBL))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette CMBL protéine est marqué à la His tag.

Fonction: Recombinant Human CMBL Protein (His Tag)

Séquence: Met 1-Met 245

Attributs du produit: A DNA sequence encoding the human CMBL (Q96DG6) (Met 1-Met 245) was expressed, with a N-terminal polyhistidine tag.

Pureté: > 97 % as determined by reducing SDS-PAGE.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 0.1 % Brij35, pH 8.0

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: CMBL (Carboxymethylenebutenolidase Homolog (CMBL))

Autre désignation: CMBL (CMBL Produits)

Synonymes: JS-1 Protein, RGD1306952 Protein, 2310016A09Rik Protein, carboxymethylenebutenolidase homolog Protein, carboxymethylenebutenolidase-like (Pseudomonas) Protein, CMBL Protein, Cmbl Protein

Sujet:

Background: Carboxymethylenebutenolidase (CMBL), also known as 4-carboxymethylenebut-2-en-4-olide lactonohydrolase, maleylacetate enol- lactonase, dienelactone hydrolase, and carboxymethylene butenolide hydrolase, is a hydrolase specially belonging to the family of hydrolases. It maily acts on carboxylic ester bonds. CMBL is a human homolog of Pseudomonas dienelactone hydrolase involved in the bacterial halocatechol degradation pathway. The ubiquitous expression of human CMBL gene transcript in various tissues was observed. CMBL was demonstrated to be the primary olmesartan medoxomil (OM) bioactivating enzyme in the liver and intestine. The recombinant human CMBL expressed in mammalian cells was clearly shown to activate OM. The recombinant CMBL also converted other prodrugs having the same ester structure as OM, faropenem medoxomil and lenampicillin, to their active metabolites. CMBL exhibited a unique sensitivity to chemical inhibitors, thus, being distinguishable from other known esterases.  

Synonym: JS-1

Poids moléculaire: 30 kDa

UniProt: Q96DG6