CD2 Protein (CD2) (His tag)

N° du produit ABIN7194720

Cette protéine Recombinant CD2 est produite dans HEK-293 Cells.

Aperçu rapide pour CD2 Protein (CD2) (His tag) (ABIN7194720)

Antigène: CD2

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Application: ELISA

Pureté: > 97 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette CD2 protéine est marqué à la His tag.

Fonction: Recombinant Human CD2 Protein (His Tag)

Séquence: Met 1-Asp 209

Attributs du produit: A DNA sequence encoding the human CD2 (NP_001758.2) extracellular domain (Met 1-Asp 209) was expressed, fused with a polyhistidine tag at the C-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized human CD2-His at 10 μg/ml (100 μl/well) can bind human CD58-Fc, The EC₅₀ of human CD58-Fc is 0.04-0.1 μg/ml.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: CD2

Autre désignation: CD2

Sujet: Erythrocyte receptor,LFA-2,LFA-3 receptor,Rosette receptor,SRBC,T-cell surface antigen CD2,T-cell surface antigen T11/Leu-5,T11,T-cell surface antigen CD2, also known as T-cell surface antigen T11/Leu-5, and SRBC, is a single-pass type I membrane protein. It contains one Ig-like C2-type domain and one Ig-like V-type domain. CD2 is a cell adhesion molecule expressed on T cells and is recognized as a target for CD48 (rats) and CD58 (humans). CD2 has been shown to set quantitative thresholds in T cell activation both in vivo and in vitro. Further, intracellular CD2 signaling pathways and networks are being discovered by the identification of several cytosolic tail binding proteins. CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain of CD2 is implicated in the signaling function. The complex of CD2 and CD58 also plays an important role in enhancing the adhesion of T lymphocytes to target cells, and promoting hyperplasia and activation of T lymphocytes. As a cell surface glycoprotein, CD2 expressed on most human T cells and natural killer (NK) cells and plays an important role in mediating cell adhesion in both T-lymphocytes and in signal transduction.

Poids moléculaire:

Calculated MW: 22.7 kDa

Observed MW: 37 kDa

ID gène: 914

NCBI Accession: NP_001758

UniProt: P06729