PD-L1 Protein (AA 19-238) (His tag)

N° du produit ABIN7320750

Cette protéine Recombinant PD-L1 est produite dans HEK-293 Cells.

Aperçu rapide pour PD-L1 Protein (AA 19-238) (His tag) (ABIN7320750)

Antigène: PD-L1 (CD274 (PD-L1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: HEK-293 Cells

Application: ELISA

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 19-238

Purification/Conjugué: Cette PD-L1 protéine est marqué à la His tag.

Fonction: Recombinant Mouse PD-L1/B7-H1/CD274 Protein (His Tag)

Séquence: Phe19-Thr238

Attributs du produit: Recombinant Mouse Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr238 is expressed with a 6His tag at the C-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized Anti-Human PDL1 mAb-Fc at 2μg/ml (100 μl/well)can bind Mouse PD-L1-His. The ED₅₀ of Mouse PD-L1-His is 23.35 ng/ml.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: PD-L1 (CD274 (PD-L1))

Autre désignation: PD-L1/B7-H1/CD274

Sujet: B7 homolog 1,B7-H1,B7h1,CD274,PD-L1,PDCD1 ligand 1,Pdcd1l1,Pdcd1lg1,Pdl1,Programmed cell death 1 ligand 1Cd274,programmed cell death 1 ligand 1,programmed death ligand 1,Mouse Programmed cell death 1 ligand 1(Cd274,PD-L1), is a member of the growing B7 family of immune proteins.It involved in the costimulatory signal essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.B7-H1 has been identified as one of two ligands for programmed death1 (PD1), a member of the CD28 family of immunoreceptors. B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. The costimulatory function of B7-H1 is critical for enhancing maturation and differentiation of T-cells in lymphoid organs.B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN gamma stimulation. Interaction of B7-H1 with PD1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.

Poids moléculaire:

Calculated MW: 25.6 kDa

Observed MW: 39-58 kDa

ID gène: 60533

NCBI Accession: NP_068693

UniProt: Q9EP73

Pathways: Cancer Immune Checkpoints