CD33 Protein (CD33) (His tag)

N° du produit ABIN7194814

Cette protéine Recombinant CD33 est exprimée dans HEK-293 Cells.

Aperçu rapide pour CD33 Protein (CD33) (His tag) (ABIN7194814)

Antigène: CD33

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Pureté: > 90 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette CD33 protéine est marqué à la His tag.

Fonction: Recombinant Human SIGLEC3/CD33 Protein (His Tag)

Séquence: Met 1-His259

Attributs du produit: A DNA sequence encoding the human CD33 (AAH28152.1) (Met1-His259) was expressed with a C-terminal polyhistidine tag.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: CD33

Autre désignation: SIGLEC3/CD33

Sujet: CD33,Myeloid Cell Surface Antigen CD33,SIGLEC3,Sialic Acid-Binding Ig-Like Lectin 3,Siglec-3,gp67,Myeloid cell surface antigen CD33 also known as Sialic acid binding Ig-like lectin 3, CD33 antigen or Siglec-3, is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. This Single-pass type I membrane protein contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain. CD33 /Siglec-3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. CD33 /Siglec-3 preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33/Siglec-3 induces apoptosis in acute myeloid leukemia (in vitro). CD33/Siglec-3 can function as a sialic acid-dependent cell adhesion molecule and that binding can be modulated by endogenous sialoglycoconjugates when CD33 is expressed in a plasma membrane.

Poids moléculaire:

Calculated MW: 28.2 kDa

Observed MW: 40-46 kDa

ID gène: 945

UniProt: P20138