CD34 Protein (CD34) (His tag)

N° du produit ABIN7320223

Cette protéine Recombinant CD34 est produite dans HEK-293 Cells.

Aperçu rapide pour CD34 Protein (CD34) (His tag) (ABIN7320223)

Antigène: CD34

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: HEK-293 Cells

Application: Cell Culture (CC)

Pureté: > 98 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette CD34 protéine est marqué à la His tag.

Fonction: Recombinant Mouse CD34 Protein (His Tag)

Séquence: Met 1-Thr 287

Attributs du produit: A DNA sequence encoding the extracellular domain of mouse CD34 (NP_001104529.1) (Met 1-Thr 287) was expressed, with a polyhistidine tag at the C-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line. When 4 x 10⁴ cells/well are added to mouse CD34 coated plates (0.8 μg/ml, 100 μl/well), approximately > 30 % will adhere after one hour at 37 °C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: CD34

Autre désignation: CD34

Sujet: AU040960,Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias.

Poids moléculaire:

Calculated MW: 28.6 kDa

Observed MW: 50-60 kDa

NCBI Accession: NP_001104529