CD6 Protein (CD6) (His tag)

N° du produit ABIN7194912

Cette protéine Recombinant CD6 est produite dans HEK-293 Cells.

Aperçu rapide pour CD6 Protein (CD6) (His tag) (ABIN7194912)

Antigène: CD6

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Rat

Source: HEK-293 Cells

Pureté: > 95 % as determined by SDS-PAGE

Détail du produit

Purification/Conjugué: Cette CD6 protéine est marqué à la His tag.

Fonction: Recombinant Rat CD6/TP120 Protein (His Tag)(Active)

Séquence: Met1-Asn396

Attributs du produit: A DNA sequence encoding the rat CD6 (Q812A4) (Met1-Asn396) was expressed with a polyhistidine tag at the C-terminus.

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: 1. Immobilized rat CD6-His at 10 μg/mL (100 μL/well) can bind rat ALCAM-Fc3 , The EC50 of rat ALCAM-Fc3 is 19.5-45.5 ng/ml. 2. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When 8 x 104 cells/well are added to ratCD6-His coated plates (10μg/mL, 100 μL/well), approximately 39.2% will adhere after 60 minutes at 37°C.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: CD6

Autre désignation: CD6/TP120

Sujet:

Background: T-cell differentiation antigen CD6, also known as TP120 and CD6, is a single-pass type I membrane protein which contains three SRCR domains. CD6 / TP120 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6 / TP120 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM ) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6 / TP120 binds to activated leukocyte cell adhesion molecule ( CD166 ), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6 / TP120 partially associates with the TCR / CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse.

Synonym: CD6

Poids moléculaire: 42.5 kDa

UniProt: Q812A4