Chitotriosidase 1 Protein (His tag)

N° du produit ABIN7195100

Protéine Recombinant Chitotriosidase 1 exprimée dans HEK-293 Cells.

Aperçu rapide pour Chitotriosidase 1 Protein (His tag) (ABIN7195100)

Antigène: Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Pureté: > 94 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette Chitotriosidase 1 protéine est marqué à la His tag.

Fonction: Recombinant Human Chitotriosidase/CHIT1 Protein (His Tag)

Séquence: Met 1-Asn 466

Attributs du produit: A DNA sequence encoding the human CHIT1 (NP_003456.1) (Met 1-Asn 466) precursor was fused with a polyhistidine tag at the C-terminus.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 12.5 mM Tris, 75 mM NaCl, 50 % glycerol, pH 7.5

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))

Autre désignation: Chitotriosidase/CHIT1

Sujet:

Background: Chitotriosidase, also known as Chitinase-1 and CHIT1, is a member of the glycosyl hydrolase 18 family and Chitinase class II subfamily. It is a member of the mammalian chitinase family, structurally homologous to chitinases from other species, is synthesized and secreted by specifically activated macrophages. Chitotriosidase is a polymer of N-acetylglucosamine. Serum and plasma chitotriosidase activity is usually measured as the first step in diagnosis of Gaucher disease. Monitoring chitotriosidase activity is widely used during treatment of this pathology by enzyme replacement therapy. Its elevated plasma level reflects gradual intralysosomal accumulation in Gaucher cells (lipid-loaded macrophages). Macrophages overloaded by the enzyme accumulated in lysosomal material (lipids) were shown to secrete chitotriosidase, its increased expression was noted in several lysosomal storage diseases and atherosclerosis. In addition to lipid storage disorders, where Chit activity has longer been used as a marker of disease activity and therapeutic response, elevation of plasma Chit may occur in hematological disorders with storage of erythrocyte membrane breakdown products as thalassemia and different systemic infectious diseases sustained by fungi and other pathogens. Recently, increased Chit activity was demonstrated in CNS from patients with different neurological disorders. Chitotriosidase is believed to play a role in mechanisms of immunity and protection against chitin-containing pathogens.

Synonym: Chitotriosidase-1, Chitinase-1, CHIT1,CHI3,CHIT,CHITD

Poids moléculaire: 50.8 kDa

NCBI Accession: NP_003456