COCH Protein (His tag)

N° du produit ABIN7317572

Cette protéine Recombinant COCH est exprimée dans HEK-293 Cells.

Aperçu rapide pour COCH Protein (His tag) (ABIN7317572)

Antigène: COCH (Cochlin (COCH))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Pureté: > 92 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette COCH protéine est marqué à la His tag.

Fonction: Recombinant Human Cochlin/COCH Protein (His Tag)

Séquence: Glu 25-Gln 550

Attributs du produit: A DNA sequence encoding the mature form of human COCH (NP_001128530.1) (Glu 25-Gln 550) was expressed, with a polyhistidine tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: COCH (Cochlin (COCH))

Autre désignation: Cochlin/COCH

Sujet: COCH-5B2,COCH5B2,DFNA9,Cochlin, also known as COCH-5B2 and COCH, is a secreted protein which contains one LCCL domain and two VWFA domains. It is an abundant inner ear protein expressed as multiple isoforms. Its function is also unknown, but it is suspected to be an extracellular matrix component. Cochlin and type II collagen are major constituents of the inner ear extracellular matrix, and Cochlin constitutes 70 % of non-collagenous protein in the inner ear, the cochlin isoforms can be classified into three subgroups, p63s, p44s and p40s. The expression of cochlin is highly specific to the inner ear. Eleven missense mutation and one in-frame deletion have been reported in the COCH gene, causing hereditary progressive sensorineural hearing loss and vestibular dysfunction, deafness autosomal dominant type 9 (DFNA9). The co-localization of cochlin and type II collagen in the fibrillar substance in the subepithelial area indicate that cochlin may play a role in the structural homeostasis of the vestibule acting in concert with the fibrillar type II collagen bundles. Defects in COCH may contribute to Meniere disease which is an autosomal dominant disorder characterized by hearing loss associated with episodic vertigo.

Poids moléculaire:

Calculated MW: 59.4 kDa

Observed MW: 66&48&18 kDa

ID gène: 1690

NCBI Accession: NP_001128530

UniProt: O43405

Pathways: Sensory Perception of Sound