DUSP14 Protein (His-MBP)

N° du produit ABIN7317376

Cette protéine Recombinant DUSP14 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour DUSP14 Protein (His-MBP) (ABIN7317376)

Antigène: DUSP14 (Dual Specificity Phosphatase 14 (DUSP14))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 88 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette DUSP14 protéine est marqué à la His-MBP.

Fonction: Recombinant Human DUSP14/MKP-6 Protein (His & MBP Tag)

Séquence: Met 1 -His 191

Attributs du produit: A DNA sequence encoding the human DUSP14 (O95147) (Met 1 -His 191) was fused with an N-terminal polyhistidine-tagged MBP tag at the N-terminus.

Stérilité: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: DUSP14 (Dual Specificity Phosphatase 14 (DUSP14))

Autre désignation: DUSP14/MKP-6

Sujet: MKP-L,MKP6,Dual specific phosphatase 14 / MAP-kinase phophatase-6 (DUSP14 / MKP6) is a member of Dual-specificity phosphatases that is a subclass of protein tyrosine phosphatases (PTP) families that can dephosphorylate bothe phosphotyrosine and phosphoserine / phosphothreonine residues in substrates. Unlike many other DUSPs, DUSP14 only contains a catalytic domain within the C-terminal region. In signal transduction, DUSP14 has been considered as negative regulator of the mitogen-activated protein kinase (MAPK) / extracellular signal-regulated kinase 1 / 2 (ERK 1 / 2) pathway. DUSP14 phosphatase activity has been confirmed to be inhibited by PTP inhibitor IV. PTP inhibitor binds to the catalytic site of DUSP14. PTP inhibitor IV effectively and specifically inhibited DUSP14-mediated dephosphorylation of JNK, a member of the mitogen-activated protein kinase (MAPK) family through dephosphorylation of both the Ser / Thr and Tyr residues of MAPKs.

Poids moléculaire:

Calculated MW: 65.0 kDa

Observed MW: 60 kDa

ID gène: 11072

UniProt: O95147