CD74 Protein (His tag)

N° du produit ABIN7317659

Cette protéine Recombinant CD74 est exprimée dans HEK-293 Cells.

Aperçu rapide pour CD74 Protein (His tag) (ABIN7317659)

Antigène: CD74 (HLA-DR-gamma (CD74))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 80 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette CD74 protéine est marqué à la His tag.

Fonction: Recombinant Human CD74 Protein (His Tag)(Active)

Séquence: Gln 73-Met 232

Attributs du produit: A DNA sequence encoding the extracellular domain (Gln 73-Met 232) of human CD74 isoform b (NP_004346.1) was fused with a polyhistidine tag at the N-terminus.

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized human CD74 at 5 μg/ml (100 μl/well) can bind biotinylated human CTSL1 with a linear range of 3.2-400 ng/ml.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: CD74 (HLA-DR-gamma (CD74))

Autre désignation: CD74

Sujet:

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD74, also known as HLA class2 histocompatibility antigen gamma chain and HLA-DR antigens-associated invariant chain, is a polypeptide involved in the formation and transport of MHC class2 protein. CD74 is expressed by B cells, macrophages, and Reed-Sternberg cells. When MHC class 2 protein was in the rough ER, its peptide-binding cleft was blocked by CD74 to prevent it from interacting with the endogenous peptides. CD74 also serves to facilitate MHC class2's export from ER.

Synonym: DHLAG,HLADG,Ia-GAMMA,II

Poids moléculaire: 20.4 kDa

NCBI Accession: NP_004346

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints