Lacritin Protein (LACRT) (His tag)

N° du produit ABIN7318709

Cette protéine Recombinant Lacritin est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Lacritin Protein (LACRT) (His tag) (ABIN7318709)

Antigène: Lacritin (LACRT)

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette Lacritin protéine est marqué à la His tag.

Fonction: Recombinant Human Lacritin/LACRT Protein (His Tag)

Séquence: Ala19-Ala138

Attributs du produit: Recombinant Human Lacritin is produced by our E.coli expression system and the target gene encoding Ala19-Ala138 is expressed with a 6His tag at the N-terminus.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: Lacritin (LACRT)

Autre désignation: Lacritin/LACRT

Sujet:

Background: Extracellular glycoprotein lacritin (Lacritin) is a secreted protein which consists of 119 amino acids after cleavage of the N-terminal signal peptide and displays several predicted alpha helices, mostly in the C-terminal half. Lacritin is highly expressed in the lacrimal gland, localizes primarily to secretory granules and secretory fluid. Lacritin modulates lacrimal acinar cell secretion, promotes ductal cell proliferation, and stimulates signaling through tyrosine phosphorylation and release of calcium. Lacritin is thus a multifunctional prosecretory mitogen with cell survival activity. Natural or bacterial cleavage of lacritin releases a C-terminal fragment that is bactericidal. Lacritin cell targeting is dependent on the cell surface heparan sulfate proteoglycan syndecan-1 (SDC1). Binding utilizes an enzyme-regulated 'off-on' switch in which active epithelial heparanase (HPSE) cleaves off heparan sulfate to expose a binding site in the N-terminal region of syndecan-1's core protein.

Synonym: Extracellular Glycoprotein Lacritin, LACRT

Poids moléculaire: 14.6 kDa

UniProt: Q9GZZ8