LAIR2 Protéine

N° du produit ABIN7317737

Cette protéine Recombinant LAIR2 est produite dans HEK-293 Cells.

Aperçu rapide pour LAIR2 Protéine (ABIN7317737)

Antigène: LAIR2 (Leukocyte-Associated Immunoglobulin-Like Receptor 2 (LAIR2))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Application: Cell Culture (CC)

Pureté: > 93 % as determined by reducing SDS-PAGE.

Détail du produit

Fonction: Recombinant Human LAIR2/CD306 Protein

Séquence: Met 1-Pro 152

Attributs du produit: A DNA sequence encoding the human LAIR2 (NP_002279.2) (Met 1-Pro 152) was expressed and purified.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of HT-29 human colon adenocarcinoma cells. When 5 x 10⁴ cells/well are added to recombiannt human LAIR2 coated plates (50 μg/ml with 100 μl/well), > 30% will adhere after 30 minutes at 37°C. Optimal concentration depends on cell type as well as the application or research objectives.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, 0.7M NaCl, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: LAIR2 (Leukocyte-Associated Immunoglobulin-Like Receptor 2 (LAIR2))

Autre désignation: LAIR2/CD306

Sujet: CD306,LAIR-2,LAIR2,Leukocyte-Associated Immunoglobulin-Like Receptor 2,Leukocyte-associated immunoglobulin-like receptor 2 ( LAIR2 ), also known as CD306, is a 131 amino acid protein containing one lg-like C2-type domain. It is expressed as a soluble receptor exhibiting high affinity for various collagen molecules to which it binds in a hydroxyproline-dependent manner. LAIR2 is a member of the immunoglobulin superfamily and was identified by its similarity to LAIR1, an inhibitory receptor present on mononuclear leukocytes. LAIR2 is thought to be secreted and may help modulate mucosal tolerance. As a natural competitor for LAIR1, soluble LAIR2 prevents binding of human LAIR1 to collagens and LAIR1 cross-linking, thereby regulating its inhibitory potential. Accordingly, LAIR2 is suggested to perform an immunoregulatory function.

Poids moléculaire:

Calculated MW: 14.1 kDa

Observed MW: 22 kDa

ID gène: 3904

NCBI Accession: NP_002279

UniProt: Q6ISS4