KARS Protein (AA 2-597) (His tag)

N° du produit ABIN7318750

Protéine Recombinant KARS exprimée dans HEK-293 Cells.

Aperçu rapide pour KARS Protein (AA 2-597) (His tag) (ABIN7318750)

Antigène: KARS (Lysyl-tRNA Synthetase (KARS))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 2-597

Purification/Conjugué: Cette KARS protéine est marqué à la His tag.

Fonction: Recombinant Human KARS Protein (His Tag)

Séquence: Ala2-Val597

Attributs du produit: Recombinant Human Lysine--tRNA Ligase is produced by our Mammalian expression system and the target gene encoding Ala2-Val597 is expressed with a 6His tag at the C-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 20 % Glycerol, pH 8.0.

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: KARS (Lysyl-tRNA Synthetase (KARS))

Autre désignation: KARS

Sujet: KARS,KIAA0070,LysRS,Lysine--tRNA Ligase,Lysyl-tRNA Synthetase,Lysine-tRNA ligase, also known as Lysyl-tRNA synthetase, LysRS, KARS and KIAA0070, belongs to the class-II aminoacyl-tRNA synthetase family. The N-terminal cytoplasmic domain (1-65) is a functional tRNA-binding domain, which is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. KARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB).

Poids moléculaire:

Calculated MW: 69.1 kDa

Observed MW: 70-90 kDa

ID gène: 3735

UniProt: Q15046