PAK3 Protein (PAK3) (His tag)

N° du produit ABIN7317075

Protéine Recombinant PAK3 exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour PAK3 Protein (PAK3) (His tag) (ABIN7317075)

Antigène: PAK3

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Pureté: > 80 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette PAK3 protéine est marqué à la His tag.

Fonction: Recombinant Human PAK3 Protein (His Tag)(Active)

Séquence: Met 1-Arg 544

Attributs du produit: A DNA sequence encoding the human PAK3 isoform 2 (O75914-2) (Met 1-Arg 544) was fused with a polyhistidine tag at the C-terminus.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 98 nmol/min/mg using MBP as substrate.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Détail du antigène

Antigène: PAK3

Autre désignation: PAK3

Sujet:

Background: PAK3 is a member of PAK proteins, a family of serine/threonine p21-activating kinases, serve as effectors of small Rho GTPases Cdc42 and RAC and have been implicated in a wide range of biological activities. There are six mammalian PAKs which can be divided into two groups: group I PAKs (PAK1-3) and group II PAKs (PAK4-6). Although the two PAK groups are architecturally similar there are differences in their mode of regulation suggesting their cellular functions are likely to be different. Group I p21-activated kinases (PAK1/2/3) is demonstrated as ERK3/ERK4 activation loop kinases. It has been shown that group I PAKs phosphorylate ERK3 and ERK4 on Ser-189 and Ser-186, respectively, both in vitro and in vivo, and that expression of activated Rac1 augments this response. Besides regulation enzymatic activation of ERK3/ERK4, PAKs can also play roles in downstream activation of MAP kinase-activated protein kinase 5 (MK5) in vivo. Thus, the  group I PAKs act as upstream activators of ERK3 and ERK4 and unravel a novel PAK-ERK3/ERK4-MK5 signaling pathway. In clinical, PAK has been proposed as a potential therapeutic target in schwannomas.

Synonym: bPAK;CDKN1A;hPAK3;MRX30;MRX47;OPHN3;PAK3beta

Poids moléculaire: 62 kDa