Periostin Protein (POSTN) (His tag)

N° du produit ABIN7317134

Cette protéine Recombinant Periostin est produite dans HEK-293 Cells.

Aperçu rapide pour Periostin Protein (POSTN) (His tag) (ABIN7317134)

Antigène: Periostin (POSTN)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Application: Cell Culture (CC)

Pureté: > 97 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette Periostin protéine est marqué à la His tag.

Fonction: Recombinant Human Periostin/OSF-2 Protein (His Tag)

Séquence: Met 1-Gln 781

Attributs du produit: A DNA sequence encoding the human POSTN isoform 3 (NP_001129407.1) corresponding to amino acid (Met 1-Gln 781) was expressed with a C-terminal polyhistidine tag.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. When cells are added to POSTN-His coated plates (10μg/mL, 100μL/well), approximately > 60% will adhere specifically after 30 minutes at 37°C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: Periostin (POSTN)

Autre désignation: Periostin/OSF-2

Sujet: OSF-2,Osteoblast-specific factor 2,PDLPOSTN,PN,POSTN,Periostin,periostin,Periostin ( POSTN ), also known as OSF2 (osteoblast specific factor 2), is a heterofunctional secreted extracellular matrix (ECM) protein comprised of four fasciclin domains that promotes cellular adhesion and movement, as well as collagen fibrillogenesis. Postn is expressed in unique growth centers during embryonic development where it facilitates epithelial-mesenchymal transition (EMT) of select cell populations undergoing reorganization. In the adult, Postn expression is specifically induced in areas of tissue injury or areas with ongoing cellular re-organization. In the adult heart Postn is induced in the ventricles following myocardial infarction, pressure overload stimulation, or generalized cardiomyopathy. Although the detailed function of Postn is still unclear, Postn-integrin interaction is thought to be involved in tumor development. Postn is frequently overexpressed in various types of human cancers, stimulating metastatic growth by promoting cancer cell survival, invasion and angiogenesis, and can be a useful marker to predict the behavior of cancer.

Poids moléculaire:

Calculated MW: 86.0 kDa

Observed MW: 80 kDa

ID gène: 10631

NCBI Accession: NP_001129407

UniProt: Q15063