PDE9A Protein (His-GST)

N° du produit ABIN7317496

Cette protéine Recombinant PDE9A est exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour PDE9A Protein (His-GST) (ABIN7317496)

Antigène: PDE9A (phosphodiesterase 9A (PDE9A))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Pureté: > 90 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette PDE9A protéine est marqué à la His-GST.

Fonction: Recombinant Human PDE9A Protein (His & GST Tag)

Séquence: Met 1-Ala 533

Attributs du produit: A DNA sequence encoding the full length of human PDE9A (O76083-2) (Met 1-Ala 533) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 5 mM GSH, pH 7.4, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: PDE9A (phosphodiesterase 9A (PDE9A))

Autre désignation: PDE9A

Sujet: HSPDE9A2,High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A, also known as PDE9A, is a member of the cyclic nucleotide phosphodiesterase family and PDE9 subfamily. PDE9A is expressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood. Highest levels of PDE9A is in brain, heart, kidney, spleen, prostate and colon. IsoformPDE9A12is found in prostate. PDE9A mRNA is widely distributed throughout the rat and mouse brain, with the highest expression observed in cerebellar Purkinje cells.PDE9A is the only cGMP-specific PDE with significant expression in the forebrain, and as such is likely to play an important role in NO-cGMP signaling. PDE9A is highly conserved between species and is widely distributed throughout the rodent brain. PDE9A is probably involved in maintenance of low cGMP levels in cells and might play an important role in a variety of brain functions involving cGMP-mediated signal transduction. PDE9A hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. PDE9A represents a novel drug target worthy of further study.

Poids moléculaire:

Calculated MW: 89.5 kDa

Observed MW: 75 kDa

ID gène: 5152

UniProt: O76083