PKD2 Protein (His-GST)

N° du produit ABIN7317066

Cette protéine Recombinant PKD2 est exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour PKD2 Protein (His-GST) (ABIN7317066)

Antigène: PKD2 (Protein Kinase D2 (PKD2))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Pureté: > 82 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette PKD2 protéine est marqué à la His-GST.

Fonction: Recombinant Human PRKD2/PKD2 Protein (His & GST Tag)

Séquence: Met 1-Leu 878

Attributs du produit: A DNA sequence encoding the amino acid sequence (Met 1-Leu 878) of human PRKD2 (NP_057541.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be > 30 nmol/min/mg using synthetic CREBtide peptide (KRREILSRRPSYR) as substrate.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as sterile solution of 50 mM Tris, 500 mM NaCl, 0.5 mM PMSF, 10 % glycerol, pH 8.0

Agent conservateur: PMSF

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: PKD2 (Protein Kinase D2 (PKD2))

Autre désignation: PRKD2/PKD2

Sujet: HSPC187,PKD2,nPKC-D2,Serine/threonine-protein kinase D2, also known as PRKD2 and PKD2, is a cytoplasm and membrane protein which belongs to theprotein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD2 / PKD2 is widely expressed. It contains onePH domain, twophorbol-ester/DAG-type zinc fingers and oneprotein kinase domain. PRKD2 / PKD2 is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-710 and phosphorylation of Ser-706 by PKC relieves auto-inhibition by the PH domain. PRKD2 / PKD2 converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.

Poids moléculaire:

Calculated MW: 124 kDa

Observed MW: 120 kDa

ID gène: 25865

NCBI Accession: NP_057541

UniProt: Q9BZL6

Pathways: cAMP Metabolic Process, Maintenance of Protein Location, Negative Regulation of Transporter Activity