STK40 Protein (His-GST)

N° du produit ABIN7197936

Cette protéine Recombinant STK40 est produite dans Baculovirus infected Insect Cells.

Aperçu rapide pour STK40 Protein (His-GST) (ABIN7197936)

Antigène: STK40 (serine/threonine Kinase 40 (STK40))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Pureté: > 97 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette STK40 protéine est marqué à la His-GST.

Fonction: Recombinant Human STK40 Protein (His & GST Tag)

Séquence: Met 1-Lys 435

Attributs du produit: A DNA sequence encoding the human STK40 (NP_114406) (Met1-Lys435) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, 3 mM DTT, 0.5M Urea, 0.5 mM GSH, pH 8.0

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: STK40 (serine/threonine Kinase 40 (STK40))

Autre désignation: STK40

Sujet: SHIK;SgK495,STK40 localized to both the cytoplasm and the nucleus. It is ubiquitously expressed. Mechanistically, Stk40 interacts with Rcn2, which also activates Erk1/2 to induce ExEn specification in mouse ESCs. Stk40 is able to activate the Erk/MAPK pathway and induce extraembryonic-endoderm (ExEn) differentiation in mouse ESCs. Interestingly, cells overexpressing Stk40 exclusively contribute to the ExEn layer of chimeric embryos when injected into host blastocysts. In contrast, deletion of Stk40 in ESCs markedly reduces ExEn differentiation in vitro. STK40 has a central serine/threonine protein kinase domain and is homologous to TRB-3, a protein that regulates activation of MAP kinases and inhibits NFκB-mediated gene transcription. Similarly, overexpression of STK40 inhibits NFκB activation triggered by TNF and also inhibits p53-mediated transcription. There are four named isoforms of STK40 that are produced as a result of alternative splicing.

Poids moléculaire:

Calculated MW: 76.8 kDa

Observed MW: 85 kDa

ID gène: 83931

NCBI Accession: NP_114406

UniProt: Q8N2I9