SRPK1 Protein (His-GST)

N° du produit ABIN7317393

Protéine Recombinant SRPK1 exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour SRPK1 Protein (His-GST) (ABIN7317393)

Antigène: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Pureté: > 80 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette SRPK1 protéine est marqué à la His-GST.

Fonction: Recombinant Human SRPK1 Protein (His & GST Tag)

Séquence: Glu 2-Ser 655

Attributs du produit: A DNA sequence encoding the human SRPK1 (AAH38292.1) (Glu 2-Ser 655) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % Glycerol, 0.1 mMEDTA, 0.5 mM PMSF, 1 mM TCEP, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Agent conservateur: PMSF

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Autre désignation: SRPK1

Sujet: RP3-422H11.1,SFRSK1,Serine / threonine-protein kinase SRPK1, also known as SFRS protein kinase 1, Serine/arginine-rich protein-specific kinase 1, SR-protein-specific kinase 1 and SRPK1, is a cytoplasm and nucleus protein which belongs to theprotein kinase superfamily and CMGC Ser/Thr protein kinase family. Isoform 2 of SRPK1 is predominantly expressed in the testis but is also present at lower levels in heart, ovary, small intestine, liver, kidney, pancreas and skeletal muscle. Isoform 1 of SRPK1 is only seen in the testis, at lower levels than isoform 2. SRPK1 hyperphosphorylates RS domain-containing proteins such as SFRS1, SFRS2 and ZRSR2 on serine residues during metaphase but at lower levels during interphase. SRPK1 plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. SRPK1 locks onto SFRS1 to form a stable complex and processively phosphorylates the RS domain. SRPK1 appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids.

Poids moléculaire:

Calculated MW: 102 kDa

Observed MW: 120 kDa

ID gène: 6732

UniProt: Q96SB4

Pathways: Toll-Like Receptors Cascades