TWF1 Protein (His-GST)

N° du produit ABIN7317477

Cette protéine Recombinant TWF1 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour TWF1 Protein (His-GST) (ABIN7317477)

Antigène: TWF1 (Twinfilin, Actin-Binding Protein 1 (TWF1))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 84 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette TWF1 protéine est marqué à la His-GST.

Fonction: Recombinant Human TWF1/Twinfilin-1 Protein (His & GST Tag)

Séquence: Met 1-Asp 252

Attributs du produit: A DNA sequence encoding the human TWF1 isoform 4 (Q12792-4) (Met 1-Asp 252) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, 500 mM NaCl, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: TWF1 (Twinfilin, Actin-Binding Protein 1 (TWF1))

Autre désignation: TWF1/Twinfilin-1

Sujet: A6,MGC23788,MGC41876,PTK9,Twinfilin-1, also known as Protein A6, Protein tyrosine kinase 9, TWF1 and PTK9, is a cytoplasm protein which belongs to theactin-binding proteins ADF family and Twinfilin subfamily. Twinfilin-1 (TWF1 / PTK9 ) is a highly conserved actin monomer-binding protein that regulates cytoskeletal dynamics in organisms from yeast to mammals. In addition to the mammalian twinfilin-1, a second protein with approximately 65 % sequence identity to twinfilin-1 exists in mouse and humans. TWF1 / PTK9 is expressed at high levels in the colon, testis, ovary, prostate and lung. It is expressed at lower levels in the brain, bladder and heart. It is not detected in liver. TWF1 / PTK9 is an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. TWF1 / PTK9 seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.

Poids moléculaire:

Calculated MW: 57.0 kDa

Observed MW: 50 kDa

ID gène: 5756

UniProt: Q12792

Pathways: Regulation of Actin Filament Polymerization, Maintenance of Protein Location