Tyrosine Hydroxylase Protein (TH) (His tag)

N° du produit ABIN7317803

Détail du produit

Antigène: Tyrosine Hydroxylase (TH)

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette Tyrosine Hydroxylase protéine est marqué à la His tag.

Fonction: Recombinant Human TH/Tyrosine Hydroxylase Protein (His Tag)

Séquence: Pro 2-Gly 497

Attributs du produit: A DNA sequence encoding the human TH isoform 2 (P07101-3) (Pro 2-Gly 497) was fused with a polyhistidine tag at the N-terminus.

Pureté: > 94 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: Tyrosine Hydroxylase (TH)

Autre désignation: TH/Tyrosine Hydroxylase (TH Produits)

Synonymes: DYT14 Protein, DYT5b Protein, TYH Protein, The Protein, CG10118 Protein, DH65B Protein, DTH Protein, Dmel\\CG10118 Protein, Pale Protein, Ple Protein, TH Protein, Th Protein, VII Protein, dTH1 Protein, dTH65B Protein, pale/ple Protein, th Protein, dyt14 Protein, dyt5b Protein, tyh Protein, tyrosine hydroxylase Protein, pale Protein, tyrosine 3-monooxygenase Protein, tyrosine hydroxylase S homeolog Protein, TH Protein, Th Protein, ple Protein, th Protein, th.S Protein, LOC100008895 Protein

Sujet:

Background: Tyrosine hydroxylase (TH) is a rate-limiting enzyme in catecholamine synthesis. Tyrosine hydroxylase activity is modulated by protein-protein interactions with enzymes in the same pathway or the tetrahydrobiopterin pathway, structural proteins considered to be chaperones that mediate the neuron's oxidative state. It is phosphorylated at serine (Ser) residues Ser8, Ser19, Ser31 and Ser40 in vitro. The phosphorylation of tyrosine hydroxylase at Ser19 or Ser8 has no direct effect on tyrosine hydroxylase activity. As tyrosine hydroxylase (TH) catalyses the formation of L-DOPA, the rate-limiting step in the biosynthesis of DA, the Parkinson's disease (PD) can be considered as a TH-deficiency syndrome of the striatum. A direct pathogenetic role of TH has also been suggested, as the enzyme is a source of reactive oxygen species (ROS) in vitro and a target for radical-mediated oxidative injury. Recently, it has been demonstrated that L-DOPA is effectively oxidized by mammalian Tyrosine hydroxylase in vitro, possibly contributing to the cytotoxic effects of DOPA.

Synonym: DYT14,DYT5b,TYH

Poids moléculaire: 57.6 kDa

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour