USP7 Protéine

N° du produit ABIN7198568

Détail du produit

Antigène: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Fonction: Recombinant Human USP7/HAUSP Protein (aa 208-560)

Séquence: Lys 208-Glu 560

Attributs du produit: A DNA sequence encoding the amino acid sequence (Lys 208-Glu 560) of human USP7 (NP_003461.2) expressed and purified, with two additional aa (Gly & Pro) at the N-terminus.

Pureté: > 98 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, pH 8.0

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))

Autre désignation: USP7/HAUSP (USP7 Produits)

Synonymes: CG1490 Protein, Dmel\\CG1490 Protein, USP7 Protein, 2210010O09Rik Protein, AA409944 Protein, AA617399 Protein, AU019296 Protein, AW548146 Protein, C80752 Protein, Hausp Protein, HAUSP Protein, TEF1 Protein, UBP Protein, Ubiquitin-specific protease 7 Protein, ubiquitin carboxyl-terminal hydrolase 7 Protein, ubiquitin specific peptidase 7 Protein, Usp7 Protein, LOC100201254 Protein, USP7 Protein

Sujet:

Background: Ubiquitin carboxyl-terminal hydrolase 7, also known as Ubiquitin thioesterase 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin-specific-processing protease 7, USP7 and HAUSP, is a widely expressed protein which belongs to the peptidase C19 family. USP7 is a member of the family of deubiquitinating enzymes. It is involved in the regulation of stress response pathways, epigenetic silencing and the progress of infections by DNA viruses. USP7 is a protein with a cysteine peptidase core, N- and C-terminal domains required for protein-protein interactions. USP7 contributes to epigenetic silencing of homeotic genes by Polycomb (Pc). USP7 cleaves ubiquitin fusion protein substrates. It deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2. USP7 also induces TP53-dependent cell growth repression and apoptosis. USP7 has key roles in the p53 pathway whereby it stabilizes both p53 and MDM2. Herpes simplex virus type 1 (HSV-1) regulatory protein ICP0 stimulates lytic infection and the reactivation of quiescent viral genomes. ICP0 interacts very strongly with USP7. USP7-mediated stabilization of ICP0 is dominant over ICP0-induced degradation of USP7 during productive HSV-1 infection. The biological significance of the ICP0-USP7 interaction may be most pronounced in natural infection situations, in which limited amounts of ICP0 are expressed.

Synonym: HAUSP;TEF1

Poids moléculaire: 41 kDa

NCBI Accession: NP_003461