UBA1 Protein (His-GST)

N° du produit ABIN7317442

Protéine Recombinant UBA1 exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour UBA1 Protein (His-GST) (ABIN7317442)

Antigène: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Pureté: > 96 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette UBA1 protéine est marqué à la His-GST.

Fonction: Recombinant Human UBE1/UBA1 Protein (His & GST Tag)

Séquence: Ser 2-Arg 1058

Attributs du produit: A DNA sequence encoding the human UBA1 (NP_003325.2) (Ser 2-Arg 1058) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 7.4, 10 % glycerol, 0.5 mM GSH
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Autre désignation: UBE1/UBA1

Sujet: A1S9,A1S9T,A1ST,AMCX1,CFAP124,CTD-2522E6.1,GXP1,POC20,SMAX2,UBA1A,UBE1,UBE1X,UBE1, also known as UBA1, belongs to the ubiquitin-activating E1 family. UBE1 gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. It also catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Defects in UBA1 can cause spinal muscular atrophy X-linked type 2 (SMAX2), also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures.

Poids moléculaire:

Calculated MW: 146 kDa

Observed MW: 130 kDa

ID gène: 7317

NCBI Accession: NP_003325

UniProt: P22314