VRK1 Protein (His-GST)

N° du produit ABIN7317540

Cette protéine Recombinant VRK1 est produite dans Baculovirus infected Insect Cells.

Aperçu rapide pour VRK1 Protein (His-GST) (ABIN7317540)

Antigène: VRK1 (Vaccinia Related Kinase 1 (VRK1))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Pureté: > 88 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette VRK1 protéine est marqué à la His-GST.

Fonction: Recombinant Human VRK1 Protein (His & GST Tag)

Séquence: Met 1-Lys 396

Attributs du produit: A DNA sequence encoding the human VRK1 (Q99986) (Met 1-Lys 396) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: VRK1 (Vaccinia Related Kinase 1 (VRK1))

Autre désignation: VRK1

Sujet: PCH1,PCH1A,VRK1 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. Serine/threonine protein kinases are tumor suppressor that controls the activity of AMP-activated protein kinase family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. VRK1 contains 1 protein kinase domain and localizes to the nucleus. VRK1 gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. As a serine/threonine kinase, VRK1 phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1), also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy.

Poids moléculaire:

Calculated MW: 73.0 kDa

Observed MW: 65-70 kDa

ID gène: 7443

UniProt: Q99986