VEGF-165 / VEGF-A (AA 27-191) (Active) Protéine

N° du produit ABIN7319413

Cette protéine Recombinant VEGF-165 / VEGF-A est exprimée dans HEK-293 Cells.

Aperçu rapide pour VEGF-165 / VEGF-A (AA 27-191) (Active) Protéine (ABIN7319413)

Antigène: VEGF-165 / VEGF-A

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Application: ELISA

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 27-191

Fonction: Recombinant Human VEGF165/VEGFA Protein

Séquence: Ala27-Arg191

Attributs du produit: Recombinant Human VEGF165 is produced by our Mammalian expression system and the target gene encoding Ala27-Arg191 is expressed.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 0.01 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized Human VEGF165 (PKSH033475) at 10μg/ml (100 μl/well) can bind Human VEGFR1-Fc (PKSH033445). The ED₅₀ of Recombinant Human VEGFR1-Fc (PKSH033445) is 36.4 ng/ml.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Citrate, 8 % Sucrose, 4 % Mannitol, 0.05 % Tween 80, pH 4.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: VEGF-165 / VEGF-A

Autre désignation: VEGF165/VEGFA

Sujet: MVCD1,VEGF,VEGF165,VPF,Vascular Endothelial Growth Factor Isoform 165,Human Vascular endothelial growth factor (VEGF), also known as VEGF-A and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.

Poids moléculaire:

Calculated MW: 19.1 kDa

Observed MW: 18-24 kDa

ID gène: 7422

UniProt: P15692